NCTC clone 2555 (ATCC® CCL-12)

Organism: Mus musculus, mouse  / 

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 82 days
Gender male
Strain C3H/HeN
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor temperature
Karyotype model number = 56; range = 53 to 116. Bi-armed (metacentric and submetacentric) chromosomes noted in 50/50 cells.
Clinical Data
male
Antigen Expression
H-2k
Genes Expressed
H-2k
Tumorigenic Yes
Effects
Yes, in irradiated C3H/HeN mice
Virus Susceptibility Vesicular stomatitis virus
Human poliovirus 3
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor KK Sanford
Year of Origin September, 1956
References

Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477

Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507

Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880

Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337

Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776

Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340

Dupree, L. T. , et al. Influence of serum protein on determination of nutritional requirements of cells in culture, Exp. Cell Res., 28, 381 (1962)

J. Natl. Cancer Inst. 23: 1059, 1960.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477

Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507

Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880

Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337

Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776

Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340

Dupree, L. T. , et al. Influence of serum protein on determination of nutritional requirements of cells in culture, Exp. Cell Res., 28, 381 (1962)

J. Natl. Cancer Inst. 23: 1059, 1960.