Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-184™) Strain Designations: CDC 2000-3025 / Type Strain: no / Biosafety Level: 2 General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits And Restrictions View Permits Classification Enterobacteriaceae, Escherichia Strain Designations CDC 2000-3025 Application Produces Shiga toxin 1Produces verocytotoxin 1Enteric ResearchFood testing Isolation Diarrheal human stool; South Dakota, 2000 Type Strain no Biosafety Level 2 Antigenic Properties Serotype O111:H8 Product Format freeze-dried Storage Conditions Frozen: -80°C or colderFreeze-Dried: 2°C to 8°CLive Culture: See Propagation Section Preceptrol® no Type Strain no Antigenic Properties Serotype O111:H8 Comments This strain was confirmed by PCR to carry the eae and stx1 genes (stx1+, stx2-, eae+). Toxigenic Medium ATCC® Medium 18: Trypticase Soy Agar/Broth Growth Conditions Temperature: 37°CAtmosphere: Aerobic Name of Depositor NA Strockbine Special Collection NCRR Contract Isolation Diarrheal human stool; South Dakota, 2000 References Nancy A Strockbine, personal communication Permits These permits may be required for shipping this product: This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS