Filobasidiella neoformans Kwon-Chung, teleomorph (ATCC® 34873)

Strain Designations: NIH B-3501 [CBS 6900, CBS 7697, CBS 7817, CCRC 20532]  /  Product Format: freeze-dried

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Deposited As Cryptococcus neoformans (Sanfelice) Vuillemin, anamorph
Strain Designations NIH B-3501 [CBS 6900, CBS 7697, CBS 7817, CCRC 20532]
Application
Biomedical Research and Development Material
assay of eumelanin pigments RefWilliamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929 RefLiu L, et al. Catecholamine oxidative products, but not melanin, are produced by Cryptococcus neoformans during neuropathogenesis in mice. Infect. Immun. 67: 108-112, 1999. PubMed: 9864203
produces monophenol monooxygenase phenol oxidase, phenoloxidase RefIkeda R, et al. Characterization of a phenol oxidase from Cryptococcus neoformans var. neoformans. Microbiol. Immunol. 37: 759-764, 1993. PubMed: 8289682
regulation of phenol oxidase and capsular polysaccharide RefJacobson ES, Compton GM. Discordant regulation of phenoloxidase and capsular polysaccharide in Cryptococcus neoformans. J. Med. Vet. Mycol. 34: 289-291, 1996. PubMed: 8873890
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Antigenic Properties serotype D RefSchmeding KA, et al. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans). Curr. Microbiol. 5: 133-138, 1981. , RefKwon-Chung KJ. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia 68: 821-833, 1976. PubMed: 790172
Preceptrol® no
Genome Sequenced Strain

Yes

Mating Type mating type alpha RefKwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936
Comments
Genome sequencing strain (The University of Oklahoma, USA).
Smooth colony type RefKwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936
Morphology After 3 days on YM medium at 25°C, colony is cream-colored, mucoid. Cells are globose, single. No hyphae observed.
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions Temperature: 25°C to 30°C
Atmosphere: Typical aerobic
Sequenced Data
No DNA sequencing was performed in house on this product.
Morphology After 3 days on YM medium at 25°C, colony is cream-colored, mucoid. Cells are globose, single. No hyphae observed.
Name of Depositor KJ Kwon-Chung
Isolation
Single-basidiospore isolate from a cross of ATCC 28957 x ATCC 28958.
Cross References

Nucleotide (GenBank) : AF316889 Filobasidiella neoformans var. neoformans elongation factor 3 mRNA, complete cds.

References

Salas SD, et al. Effect of the laccase gene, CNLAC1, on virulence of cryptococcus neoformans. J. Exp. Med. 184: 377-386, 1996. PubMed: 8760791

Schmeding KA, et al. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans). Curr. Microbiol. 5: 133-138, 1981.

Kwon-Chung KJ. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia 68: 821-833, 1976. PubMed: 790172

Kwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936

Jacobson ES, Compton GM. Discordant regulation of phenoloxidase and capsular polysaccharide in Cryptococcus neoformans. J. Med. Vet. Mycol. 34: 289-291, 1996. PubMed: 8873890

Williamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929

Liu L, et al. Catecholamine oxidative products, but not melanin, are produced by Cryptococcus neoformans during neuropathogenesis in mice. Infect. Immun. 67: 108-112, 1999. PubMed: 9864203

Blakely G, et al. Evolutionary divergence of an elongation factor 3 from Cryptococcus neoformans. J. Bacteriol. 183: 2241-2248, 2001. PubMed: 11244063

Ikeda R, et al. Characterization of a phenol oxidase from Cryptococcus neoformans var. neoformans. Microbiol. Immunol. 37: 759-764, 1993. PubMed: 8289682

Kupfer DM, et al. Introns and splicing elements of five diverse fungi. Eukaryot. Cell 3(5): 1088-1100, 2004. PubMed: 15470237

Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334

Schmeding KA, et al. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans). Curr. Microbiol. 5: 133-138, 1981.

Kwon-Chung KJ. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia 68: 821-833, 1976. PubMed: 790172

Kwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation