Pachysolen tannophilus Boidin et Adzet (ATCC® 32691)

Strain Designations: NRRL Y-2460 [CBS 4044, CCRC 20329, IMI 344640, MUCL 31901, NCYC 614, VTT C-81121]  /  Product Format: freeze-dried

Permits and Restrictions

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Strain Designations NRRL Y-2460 [CBS 4044, CCRC 20329, IMI 344640, MUCL 31901, NCYC 614, VTT C-81121]
Bioethanol production
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes
Preceptrol® no
Genome Sequenced Strain


Enhanced ethanol tolerance
Trehalase activity
Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA).
Name of Depositor NRRL
Chain of Custody
ATCC <-- NRRL <-- J. Boidin
Tanning liquor
Cross References

Nucleotide (GenBank) : U40706 Pachysolen tannophilus aldose reductase gene, complete cds.

Nucleotide (GenBank) : LZCH00000000 Pachysolen tannophilus NRRL Y-2460, whole genome shotgun sequencing project


Bolen PL, Detroy RW. Induction of NADPH-linked D-xylose reductase and NAD-linked xylitol dehydrogenase activities in Pachysolen tannophilus by D-xylose, L-arabinose, or D-galactose. Biotechnol. Bioeng. 26: 302-307, 1985.

Ditzelmuller G, et al. Xylitol dehydrogenase from Pachysolen tannophilus. FEMS Microbiol. Lett. 25: 195-198, 1984.

Bolen PL, et al. Affinity purifications of aldose reductase and xylitol dehydrogenase from the xylose-fermenting yeast Pachysolen tannophilus. Appl. Environ. Microbiol. 52: 660-664, 1986.

Rokem JS, Zomer E. Scale up of the production of xylitol dehydrogenase and alcohol dehydrogenase from Pachysolen tannophilus. Appl. Microbiol. Biotechnol. 26: 231-233, 1987.

Kurtzman CP, et al. Conversion of D-xylose to ethanol by the yeast Pachysolen tannophilus. US Patent 4,359,534 dated Nov 16 1982

Jeffries TW. Process for enhanced fermentation of xylose to ethanol. US Patent 4,663,284 dated May 5 1987

Mahmourides, G et al. Ethanol accumulation in cultures of Pachysolen tannophilus on D-xylose is associated with a transition to a state of low oxygen consumption. Bio-Technology 3: 59-62, 1985.

Boidin J, Adzet JM. Deux curieuses levures isolees d'extraits tannants d'origine vegetale: Pachysolen (nov. gen.) tannophilus nov. sp. et P. pelliculatus nov. sp.. Bull. Soc. Mycol. Fr. 73: 331, 1957.

Kavanagh K, Whittaker PA. Application of the Melle-Boinot process to the fermentation of xylose by Pachysolen tannophilus. Appl. Microbiol. Biotechnol. 42: 28-31, 1994.

Vanetti MC, Aquarone E. Riboflavin excretion by Pachysolen tannophilus grown in synthetic medium supplemented with D-xylose. World J. Microbiol. Biotechnol. 8: 190-191, 1992.

Soto T, et al. Posttranslational regulatory control of trehalase induced by nutrients, metabolic inhibitors, and physical agents in Pachysolen tannophilus. Fungal Genet. Biol. 20: 143-151, 1996.

Maleszka R, et al. Ethanol production from D-galactose and glycerol by Pachysolen tannophilus. Enzyme Microb. Technol. 4: 349-352, 1982.

Schneider H, et al. Conversion of D-xylose into ethanol by the yeast Pachysolen tannophilus. Biotechnol. Lett. 3: 89-92, 1981.

Maleszka R, et al. Enhanced rate of ethanol production from D-xylose using recycled or immobilized cells of Pachysolen tannophilus. Biotechnol. Lett. 3: 415-420, 1981.

Ohno N, et al. Purification and properties of an exo(1 --->3)-beta-D-glucanase from the culture filtrate of Mucor hiemalis. Carbohydr. Res. 158: 217-226, 1986.

Kurtzman CP. Biology and physiology of the D-xylose fermenting yeast Pachysolen tannophilus. Adv. Biochem. Eng. Biotechnol. 27: 73-83, 1983.

Slininger PJ, et al. Conversion of D-xylose to ethanol by the yeast Pachysolen tannophilus. Biotechnol. Bioeng. 24: 371-384, 1982.

Detroy RW, et al. Bioconversion of wheat straw cellulose/ hemicellulose to ethanol by Saccharomyces uvarum and Pachysolen tannophilus. Biotechnol. Bioeng. 24: 1105-1113, 1982.

Riley R, et al. Comparative genomics of biotechnologically important yeasts. Proc Natl Acad Sci USA 2016 Aug 17. pii: 201603941. PubMed: 27535936

Kurtzman CP, Robnett CJ. Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species. FEMS Yeast Res. 13: 23-33, 2013. PubMed: 22978764

Liu X, Mortensen UH, Workman M. Expression and functional studies of genes involved in transport and metabolism of glycerol in Pachysolen tannophilus. Microb Cell Fact 12: 27, 2013. PubMed: 23514356

Liu X, Kaas RS, Jensen PR, Workman M. Draft genome sequence of the yeast Pachysolen tannophilus CBS 4044/NRRL Y-2460. Eukaryot Cell 11: 827, 2012. PubMed: 22645232

Kurtzman CP, Robnett CJ. Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. FEMS Yeast Res 10: 353-361, 2010. PubMed: 20522116

Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4): 331-371, 1998. PubMed: 9850420

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation