Yarrowia lipolytica (Wickerham et al.) van der Walt et von Arx (ATCC® 20460)

Alternate State: Candida lipolytica (Harrison) Diddens et Lodder  /  Strain Designations: IFP29 [CBS 7504, W29, CLIB 89]  /  Product Format: freeze-dried

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Deposited As Endomycopsis lipolytica Wickerham et al.
Strain Designations IFP29 [CBS 7504, W29, CLIB 89]
Synonym Endomycopsis lipolytica Wickerham et al., Saccharomycopsis lipolytica (Wickerham et al.) Yarrow
Alternate State Candida lipolytica (Harrison) Diddens et Lodder
Application
Produces citric acid citrate
Produces isocitric acid isocitrate
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Genome Sequenced Strain

Yes

Mating Type mating type A, mates with ATCC 18944 RefGaillardin CM, et al. A study of copulation, sporulation and meiotic segregation in Candida lipolytica. Arch. Mikrobiol. 92: 69-83, 1973. PubMed: 4725824
Comments
Aconitase
Genome sequencing strain (Genolevures Consortium, France).
Morphology After 5 days on YM media colonies butyrous to mycelial and tannish-white in color. Cells are spheroidal, ellipsoidal to elongate and single, in pairs or small clusters. Pseudohyphae and true hyphae are usually present.
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions Temperature: 20°C to 25°C
Atmosphere: Typical aerobic 
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

TTGATTTTATCTATTTCTGTGGATTTCTGGTATATTACAGCGTCATTTTATCTCAATTATAACTATCAACAACGGATCTCTTGGCTCTCACATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACTTGCAGATGTGAATCATCAATCTTTGAACGCACATTGCGCGGTATGGCATTCCGTACCGCACGGATGGAGGAGCGTGTTCCCTCTGGGATCGCATTGCTTTCTTGAAATGGATTTTTTTAAACTCTCAATTATTACGTCATTTCACCTCC


D1D2 region of the 28S ribosomal RNA gene

ACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAACCCTCGGGATTGTAATTTGAAGATTTGGCATTGGAGAAAGCTAACCCAAGTTGCTTGGAATAGTACGTCATAGAGGGTGACAACCCCGTCTGGCTAACCGTTCTCCATGTATTGCCTTATCAAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAGTGGGTGGTAAACTCCATCTAAAGCTAAATACTGGTGAGAGACCGATAGCGAACAAGTACTGTGAAGGAAAGGTGAAAAGAACTTTGAAAAGAGAGTGAAATAGTATGTGAAATTGTTGATAGGGAAGGAAATGAGTGGAGAGTGGCCGAGGTTTCAGCCGCCCCTCGTGGGCGGTGTACTGCCGACGCCGAGTCATCGATAGCGAGACGAGGGTTACAAATGGGAGCGCCTTCGGGCGTTCTCCCCTAACCCTCCACACTGCCACCGACGACATAAT

Morphology After 5 days on YM media colonies butyrous to mycelial and tannish-white in color. Cells are spheroidal, ellipsoidal to elongate and single, in pairs or small clusters. Pseudohyphae and true hyphae are usually present.
Name of Depositor C Gaillardin
U.S. Patent Number
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Isolation
Soil
Cross References

Nucleotide (GenBank) : KU729095 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : X65225 Y.lipolytica PHO2 gene for acid phosphatase.

Nucleotide (GenBank) : AJ006754 Yarrowia lipolytica URA5-SEC65 gene region.

Nucleotide (GenBank) : Z34956 Y.lipolytica HOY1 gene for DNA-binding protein.

Nucleotide (GenBank) : Z49114 Y.lipolytica LYS1 gene for homocitrate synthase.

Nucleotide (GenBank) : Z22571 Y.lipolytica orotate phosphoribosyl transferase gene.

Nucleotide (GenBank) : Z23265 Y.lipolytica CRF1 gene encoding product required for copper.

Nucleotide (GenBank) : Z22570 Y.lipolytica signal recognition particle protein (SRP19) gene.

Nucleotide (GenBank) : Z46872 Y.lipolytica gene for exo-1,3-beta-glucanase/1,3-beta-D-glucan.

Nucleotide (GenBank) : Z23264 Y.lipolytica MTP1 and MTP2 genes encoding metallothionein-I and metallothionein-II.

Nucleotide (GenBank) : KC585416 Large subunit ribosomal RNA gene, partial sequence

Nucleotide (GenBank) : LJBI00000000 Yarrowia lipolytica strain W29, whole genome shotgun sequencing project

References

Maldonado P, Charpentier M. Fermentation process for the production of citric and isocitric acids. US Patent 3,997,399 dated Dec 14 1976

Gaillardin CM, et al. A study of copulation, sporulation and meiotic segregation in Candida lipolytica. Arch. Mikrobiol. 92: 69-83, 1973. PubMed: 4725824

Treton B, Heslot H. Etude de quelques proprietes de l'aconitase de la levure Saccharomycopsis lipolytica. Agric Biol Chem 42: 1201-1206, 1978.

French Patent 72/41 913

Souciet JL, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies. FEBS Lett. 487: 3-12, 2000.

Casaregola S, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 17. Yarrowia lipolytica. FEBS Lett. 487: 95-100, 2000.

Knutsen AK, et al. Polyphasic re-examination of Yarrowia lipolytica strains and the description of three novel Candida species: Candida oslonensis sp. nov., Candida alimentaria sp. nov. and Candida hollandica sp. nov. Int. J. Syst. Evol. Microbiol. 57: 2426-2435, 2007. PubMed: 17911320

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262

Fickers P, et al. Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytica. Fungal Genet. Biol. 42: 264-274, 2005. PubMed: 15707847

Butler G, et al. Evolution of the MAT locus and its Ho endonuclease in yeast species. Proc. Natl Acad. Sci. USA 101: 1632-1637, 2004. PubMed: 14745027

Casaregola S, et al. Ylli, a non-LTR retrotransposon L1 family in the dimorphic yeast Yarrowia lipolytica. Mol. Biol. Evol. 19: 664-677, 2002. PubMed: 11961100

Kerscher S, et al. The complete mitochondrial genome of Yarrowia lipolytica. Comp. Funct. Genomics 2: 80-90, 2001. PubMed: 18628906

Mauersberger S, et al. Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization. J. Bacteriol. 183: 5102-5109, 2001. PubMed: 11489863

Richard M, et al. Tagging morphogenetic genes by insertional mutagenesis in the yeast Yarrowia lipolytica. J. Bacteriol. 183: 3098-3107, 2001. PubMed: 11325938

Vernis L, et al. Only centromeres can supply the partition system required for ARS function in the yeast Yarrowia lipolytica. J. Mol. Biol. 305: 203-217, 2001. PubMed: 11124900

Pomraning KR, Baker SE. Draft genome sequence of the dimorphic yeast Yarrowia lipolytica strain W29. Genome Announc 3: e01211-15, 2015. PubMed: 26607882

Gaillardin CM, et al. A study of copulation, sporulation and meiotic segregation in Candida lipolytica. Arch. Mikrobiol. 92: 69-83, 1973. PubMed: 4725824

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