Pseudallescheria angusta (Malloch et Cain) McGinnis et al. (ATCC® MYA-3634)

Strain Designations: SC1  /  Product Format: frozen

Deposited As Scedosporium apiospermum
Strain Designations SC1
Application
Antifungal susceptibility testing RefGhannoum MA, et al. Interlaboratory study of quality control isolates for a broth microdilution method (modified CLSI M38-A) for testing susceptibilities of dermatophytes to antifungals. J. Clin. Microbiol. 44: 4353-4356, 2006. RefEspinel-Ingroff A, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J. Clin. Microbiol. 43: 5243-5246, 2005.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Comments This fungus was re-identified as Pseudallescheria angusta based on the multigene DNA sequence comparison.
Morphology After 5 days cleistothecia form, immersed, membranaceous, spherical, light brown to black. Erect synnemata may be present, producing broadly clavate, subhyaline or pale brown conidia. Conidiogenous cells also arising from undifferentiated hyphae, cylindrical, producing slimy heads of 1-celled, smooth-walled, subhyaline to brown, subspherical to elongate conidia.
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACAGAGTTACTACTCCAAACCCATTGTGAACCTTACCTATGTTCTGTTGCCTCGGCGGCGTGGTCAGCGCCCCTCTGAAAAGAGGACGATGCCCTCCCGCCGGCAGCACCAAACTCTTGAATTTTACAGCGGATTACAATTCTGATTTGAAAACAAAAAACAAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGGCAGTAATCTGCCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCTCCGTTTCCTTAGGGAAGCTCAGGGTCGGCGTTGGGGCGCTACGGCGAGTCTTCGCGACCCCCGTAGGCCCTGAAATACAGTGGCGGTCCCGCCGCGGTTGCCTTCTGCGTAGTAAGTCTCTTTTGCAAGCTCGCATTGGGTCCCGGCGGAGGCCTGCCGTCAAACCTATAACTTCAGATGGTTTGACCTCGGATCAGGTAGGGTTACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGGCCTCTGGGCCGTCCGAGTTGTAATTTGAAGAGGATGCTTTTGGCGAGGTGCCTTCCGAGTGCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTATGGTTGGACGCCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTAAACCCCTTCTAAAGCTAAATACTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTTGTGCTCGTCGAATCAGCCGCCGCTCGTCGGCGGCGCACTTCGGCGGGCTCAGGCCAGCATCAGTTCGCTGCAGGGGGAGAAAGGCGATGGGAATGTGGCTCTTCGGAGTGTTATAGCCCGCCGCGCAATACCCCTCGGCGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCGTCAGCGAC


Beta-tubulin gene (bTub)

CCCCCCTCCCATTCCGAGCTCGCGATATATCCTATCTCAGGATCCATCTCGACCTTCATCATCGTCACTCGATGCTCTTTTGCCATCGTCTGGCGGTGAAGCTGTCGTCGGGCAATATTGCGACGCGTTCAAGCTTGCCTCCTTCAACGGCGGTGCACTTGGTGACCCCGCCCACAGGACGAGACATAGCTTGAAGAGATGCAGCCTCGTCCAATAAACCATCCAATGGGTTAAGTTTCGAATGCTAACGTATATTTTCTTTTTCCTCGTGTACAGGTTCATCTGCAGACAGGCCAGTGCGTAAGTAAATTCTTGAGGACCCTATGTCCTTTTCCCCCCTGCTTCCATTTCCCTTCTGGGCCATGATCTTGTCTGCGGGGCTAACGTTTATTTTTGCCCCCCTTTCTAGGGAAACCAAATTGGTGCTGCCTTCTGGTGCGTACATTTTTCCCGACGACGCGCGATCGAGAAACGGATGCGAATATAATTCTTCTGTGCGAGATATTTCTATTTGATGGACAGAGTACTTATCGTATTTTTAGGCAAACCATTTCTGGCGAGCACGGTCTTGATAGCAACGGAGTGTACGGAACCCCCTCATCCCTTTCCCCCCCAAATGCCAGTCACATCGACTGGTGCTGCTCTCACATTCACGGCAGACACTGATTGTCCCTTATACGTCAAAGTTACAATGGCACTTCTGAACTCCAGCTTGAGCGCATGAGCGTCTACTTCAACGAGGTAAGTTTTTGGTTCAAACCATGGGCTGGTTGGTGTGGTGTCATCCGGCCTCCGTTGTTGCAGAAAATACTAACGTATTGGTTACCTCCACATTATAGGCTTCGGGCAACAAATATGTTCCTCGCGCTGTCCTTGTAGATCTCGAGCCCGGTACCATGGACGCCGTCCGT

Morphology After 5 days cleistothecia form, immersed, membranaceous, spherical, light brown to black. Erect synnemata may be present, producing broadly clavate, subhyaline or pale brown conidia. Conidiogenous cells also arising from undifferentiated hyphae, cylindrical, producing slimy heads of 1-celled, smooth-walled, subhyaline to brown, subspherical to elongate conidia.
Name of Depositor TJ Walsh
Special Collection NSF - Mycology
Chain of Custody
ATCC <-- T J Walsh
Isolation
Human heart
References

Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi: Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M38-A2.

Ghannoum MA, et al. Interlaboratory study of quality control isolates for a broth microdilution method (modified CLSI M38-A) for testing susceptibilities of dermatophytes to antifungals. J. Clin. Microbiol. 44: 4353-4356, 2006.

Espinel-Ingroff A, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J. Clin. Microbiol. 43: 5243-5246, 2005.