alpha BL5C2.870016 [870016, aBL5C2.870016] (ATCC® HB-9909)

Organism: bovine (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism bovine (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Derivation
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Initial hybrids were refused with additional lymph node cells from the same animal and reselected.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1)
Comments
Animals were immunized with live BHV-1.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Initial hybrids were refused with additional lymph node cells from the same animal and reselected.
The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
horse serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 85%; DMSO, 15%
Isotype IgG1
Name of Depositor The United States of America
References

Levings RL, Stoll IR. Bovine monoclonal antibodies to bovine herpesvirus I from sequential fusion heterohybridomas. US Patent 5,026,646 dated Jun 25 1991

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Levings RL, Stoll IR. Bovine monoclonal antibodies to bovine herpesvirus I from sequential fusion heterohybridomas. US Patent 5,026,646 dated Jun 25 1991