mAb 13-1 (ATCC® HB-10565)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain: BALB/c (B cell); unknown (myeloma)

Applications
The antibody binds an epitope comprising amino acids 729-736 of human CFTR.
The antibody can be used to purify CFTR from an impure solution containing CFTR, to detect CFTR in a biological sample, and to determine the orientation of CFTR in the membrane of a lipid vesicle.
Derivation
Spleen cells were fused with mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the cystic fibrosis transmembrane conductance regulator (CFTR)
Comments
Animals were immunized with a fusion protein comprising amino acids 591-829 (from exon 13) of the human cystic fibrosis transmembrane conductance regulator (CFTR) and beta galactosidase.
Spleen cells were fused with mouse myeloma cells.
The antibody binds an epitope comprising amino acids 729-736 of human CFTR.
The antibody can be used to purify CFTR from an impure solution containing CFTR, to detect CFTR in a biological sample, and to determine the orientation of CFTR in the membrane of a lipid vesicle.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium 90%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor Genzyme Corp.
References

Cheng SH, et al. Antibodies specific for cystic fibrosis transmembrane conductance regulator and uses therefor. US Patent 5,981,714 dated Nov 9 1999

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Cheng SH, et al. Antibodies specific for cystic fibrosis transmembrane conductance regulator and uses therefor. US Patent 5,981,714 dated Nov 9 1999