Derivation
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Spleen cells were fused with Sp2/0 mouse myeloma cells.
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Genes Expressed
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immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain
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Cellular Products
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immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain
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Effects
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Yes, forms ascites in BALB/c mice
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Comments
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Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362). The LHR-29 ( ATCC CRL-2685), LHR-74 ( ATCC CRL-2686) and LHR-1055 ( ATCC CRL-2687) antibodies are additive. They recognize different epitopes. The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.
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