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LHR-74 (ATCC^® CRL-2686^™)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte /

LHR-74 ATCC^® CRL-2686^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits and Restrictions	View Permits
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1
Applications	The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.

Derivation	Spleen cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain
Tumorigenic	Yes
Effects	Yes, forms ascites in BALB/c mice
Comments	Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362). The LHR-29 (ATCC CRL-2685), LHR-74 (ATCC CRL-2686) and LHR-1055 (ATCC CRL-2687) antibodies are additive. They recognize different epitopes. The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.

Complete Growth Medium	Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing	Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C

Isotype	IgG1; kappa light chain

Name of Depositor	H Loosfelt, E Milgrom
References	Meduri G, et al. Luteinizing hormone/human chorionic gonadotropin receptors in breast cancer. Cancer Res. 57: 857-864, 1997. PubMed: 9041186

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
References	Meduri G, et al. Luteinizing hormone/human chorionic gonadotropin receptors in breast cancer. Cancer Res. 57: 857-864, 1997. PubMed: 9041186