anti-SR (1H4) (ATCC® CRL-2383)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type:: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the family of 6 essential splicing factors, the SR proteins.
Specificity is similar to Mab104 (ATCC CRL-2067) but because it is an IgG may be potentially more useful than Mab104 which is an IgM.
This antibody may be useful to determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in Western Blot, immunoprecipitation, and cytological assays.
Derivation
Animals were immunized with mass-isolated nuclei from the oocytes of Xenopus laevis.
Spleen cells were fused with FOX-NY myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors)
Cellular Products
immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors)
Comments
Animals were immunized with mass-isolated nuclei from the oocytes of Xenopus laevis.
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with the family of 6 essential splicing factors, the SR proteins.
SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins).
The family consists of at least 6 proteins with approximate masses of 75000, 55000, 40000, two at 30000 and 20000 daltons.
Specificity is similar to Mab104 (ATCC CRL-2067) but because it is an IgG may be potentially more useful than Mab104 which is an IgM.
This antibody may be useful to determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in Western Blot, immunoprecipitation, and cytological assays.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.075 mM adenine, 800 nM aminopterin, 0.016 mM thymidine (AAT) and 20% fetal bovine serum
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternately, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor KM Neugebauer, MB Roth
References

Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396

Basic Documentation
References

Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396