anti-SR (1H4) (ATCC® CRL-2383)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the family of 6 essential splicing factors, the SR proteins.
Specificity is similar to Mab104 (ATCC CRL-2067) but because it is an IgG may be potentially more useful than Mab104 which is an IgM.
This antibody may be useful to determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in Western Blot, immunoprecipitation, and cytological assays.
Derivation
Animals were immunized with mass-isolated nuclei from the oocytes of Xenopus laevis.
Spleen cells were fused with FOX-NY myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors)
Cellular Products
immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors)
Comments
Animals were immunized with mass-isolated nuclei from the oocytes of Xenopus laevis.
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with the family of 6 essential splicing factors, the SR proteins.
SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins).
The family consists of at least 6 proteins with approximate masses of 75000, 55000, 40000, two at 30000 and 20000 daltons.
Specificity is similar to Mab104 (ATCC CRL-2067) but because it is an IgG may be potentially more useful than Mab104 which is an IgM.
This antibody may be useful to determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in Western Blot, immunoprecipitation, and cytological assays.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.075 mM adenine, 800 nM aminopterin, 0.016 mM thymidine (AAT) and 20% fetal bovine serum
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternately, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor KM Neugebauer, MB Roth
References

Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396