YN1/1.7.4 (ATCC® CRL-1878)

Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)  /  Cell Type:: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a 95000 to 100000 dalton antigen (ICAM-1) on activated and proliferating murine lymphocytes.
Derivation
Spleen cells were fused with Y3 rat myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine ICAM-1 (ICAM 1, intercellular adhesion molecule)
Cellular Products
immunoglobulin; monoclonal antibody; against murine ICAM-1 (ICAM 1, intercellular adhesion molecule)
Comments
Animals were immunized with NS-1 mouse myeloma cells.
Spleen cells were fused with Y3 rat myeloma cells.
The antibody reacts with a 95000 to 100000 dalton antigen (ICAM-1) on activated and proliferating murine lymphocytes.
The antibody partially inhibits Con A stimulation of spleen cells but does not inhibit LPS stimulation.
The antibody strongly inhibits mixed lymphocyte responses (MLR).
The ICAM-1 antigen is absent on most unstimulated thymocytes and spleen cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml.
Isotype IgG2b
Name of Depositor F Takei
References

Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2). J. Immunol. 134: 1403-1407, 1985. PubMed: 2981916

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2). J. Immunol. 134: 1403-1407, 1985. PubMed: 2981916