BC9-E5 (ATCC® CRL-1670)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type:: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen)
Comments
Mice were immunized with naked Salmonella minnesota to which had been adsorbed neutral glycolipids from human Type A meconium.
Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor WW Young
References

Young WW Jr., et al. Characterization of monoclonal antibodies specific for the Lewis a human blood group determinant. J. Biol. Chem. 258: 4890-4894, 1983. PubMed: 6833281

Galanos C, et al. Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides. Eur. J. Biochem. 24: 116-122, 1971. PubMed: 4944989

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Young WW Jr., et al. Characterization of monoclonal antibodies specific for the Lewis a human blood group determinant. J. Biol. Chem. 258: 4890-4894, 1983. PubMed: 6833281

Galanos C, et al. Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides. Eur. J. Biochem. 24: 116-122, 1971. PubMed: 4944989