Porcine respiratory coronavirus (ATCC® VR-2384)

Classification: Coronaviridae, Alphacoronavirus, Alphacoronavirus 1  /  Product Format: frozen 1.0 mL

Classification Coronaviridae, Alphacoronavirus, Alphacoronavirus 1
Agent Porcine respiratory coronavirus
Strain AR310
Applications
This strain provides a biological pure culture of a novel pathogenic porcine respiratory coronavirus (PRCV) and a vaccine derived therefrom effective against PRCV infection and transmissible gastroenteritis virus (TGEV) infection.
The virus produces moderate pneumonia in pigs, there is no indication of human health hazard.
Biosafety Level 2
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-2384 images
Comments

This strain provides a biological pure culture of a novel pathogenic porcine respiratory coronavirus (PRCV) and a vaccine derived therefrom effective against PRCV infection and Transmissible gastroenteritis virus (TGEV) infection.  The virus produces a moderate pneumonia in pigs.  There is no indication of human health hazard.

This material is cited in U.S. and/or other patent # 5,419,907 May 30, 1995.

Effect on Host
CPE, fused and rounded cells with sloughing
Recommended Host
ST cells (ATCC® CRL-1746™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 2-3 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, fused and rounded cells with sloughing
Name of Depositor Iowa State University Research Foundation
Special Collection ATCC
Source Culture of a novel TGEV-related Porcine respiratory coronavirus
References

Paul PS, et al. Pathogenic porcine respiratory coronavirus. US Patent 5,419,907 dated May 30 1995