NCI-H128 [H128] (ATCC® HTB-120)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: pleural effusion  /  Disease: carcinoma; small cell lung cancer

Organism Homo sapiens, human
Tissue lung; derived from metastatic site: pleural effusion
Product Format frozen
Morphology floating aggregates
Culture Properties floating aggregates
Biosafety Level 1
Disease carcinoma; small cell lung cancer
Age 60 years
Gender male
Ethnicity Black
Applications
It retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.
Karyotype There are 2 distinct aneuploid peaks, both have characteristic 3p deletion.
Clinical Data
60 years
Black
male
Tumorigenic Yes
Effects
Yes, in nude mice
Comments

This cell line is aneuploid.

Will form colonies in soft agar.

It retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.

NCI-H128 cells do well on a rotary shaker flask at 70 to 80 rpm at 37°C.

It is normal for cultures of this line to have fairly large amounts of cell debris.

Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20% - OR - Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing

Allow cell aggregates to settle to the bottom of the flask, discard the supernatant medium, disperse the cells with gentle pipetting and dispense into new flasks. Subculture every 6 to 8 days.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 90%; DMSO, 10%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 13
D5S818: 9
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, A
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1
Name of Depositor AF Gazdar
References

Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

Adi F, et al. Establishment of Continuous, Clonable Cultures of Small-Cell Carcinoma of the Lung Which Have Amine Precursor Uptake and Decarboxylation Cell Properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258

Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

The cells form tumors with typical small cell carcinoma histology.

Cross References

Nucleotide (GenBank) : M22491 Human bone morphogenetic protein-3 (BMP-3) mRNA.

Basic Documentation
References

Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

Adi F, et al. Establishment of Continuous, Clonable Cultures of Small-Cell Carcinoma of the Lung Which Have Amine Precursor Uptake and Decarboxylation Cell Properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258

Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

The cells form tumors with typical small cell carcinoma histology.