Hs 895.T (ATCC® CRL-7637)

Organism: Homo sapiens, human  /  Cell Type: fibroblast,Melanoma  / 

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Organism Homo sapiens, human
Cell Type fibroblast,Melanoma
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 48 years
Gender female
Ethnicity Caucasian
Applications
Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Derivation
This melanoma cell line was isolated from the same patient as Hs 895.Sk (ATCC CRL-7636), a normal skin fibroblast. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Clinical Data
This melanoma cell line was isolated from the same patient as Hs 895.Sk (ATCC CRL-7636), a normal skin fibroblast.
female
Caucasian
48 years
Comments
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
This melanoma cell line was isolated from the same patient as Hs 895.Sk (ATCC CRL-7636), a normal skin fibroblast. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12,14
D5S818: 11,12
D7S820: 10,12
THO1: 6,7
TPOX: 8,11
vWA: 18
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation