NCI-BL1437 [BL1437] (ATCC® CRL-5958™) Organism: Homo sapiens, human / Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed / Tissue: peripheral blood / Disease: Adenocarcinoma General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Restrictions Organism Homo sapiens, human Tissue peripheral blood Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 2 [Cells Contain Epstein Barr Virus (EBV) a Herpesvirus] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease Adenocarcinoma Age 60 years Gender male Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Comments A lung adenocarcinoma cell line from this patient is available as ATCC CRL-5872. Complete Growth Medium HITES medium supplemented with 5% fetal bovine serumThe base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.) Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 to 5 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal: Every two to three days (depending on cell density). Cryopreservation Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. Culture Conditions Temperature: 37°C STR Profile Amelogenin: X,YCSF1PO: 11,12D13S317: 12D16S539: 11,12D5S818: 11,14D7S820: 8,11THO1: 6,8TPOX: 9,10vWA: 17,18 Name of Depositor AF Gazdar, JD Minna References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Basic Documentation Product Sheet Certificate of Analysis SDS Restrictions The line is available with the following restrictions: This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.