5C6 Clone 1 (ATCC® CRL-1969)

Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the mouse myelomonocytic adhesion promoting complement receptor (CR3, CD11b).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Y3 myeloma cells.
Clinical Data
It inhibits myelomonocytic adhesion in vitro, and recruitment of inflammatory cells in vivo.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD11b (type 3 complement receptor, CR3)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD11b (type 3 complement receptor, CR3)
Comments

mAb 5C6 was the product of a fusion between spleen cells from an AO rat, immunized four times with TPM, and fused with the Y3 rat myeloma line.

The antibody reacts with the mouse myelomonocytic adhesion promoting complement receptor (CR3, CD11b).
It inhibits myelomonocytic adhesion in vitro, and recruitment of inflammatory cells in vivo.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation 50% FBS + 40% complete growth medium + 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor H Rosen, S Gordon
References

Rosen H. Role of CR3 in induced myelomonocytic recruitment: insights from in vivo monoclonal antibody studies in the mouse. J. Leukocyte Biol. 48: 465-469, 1990. PubMed: 2230596

Hutchings P, et al. Transfer of diabetes in mice prevented by blockade of adhesion- promoting receptor on macrophages. Nature 348: 639-642, 1990. PubMed: 2250718

Rosen H, Gordon S. Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J. Exp. Med. 166: 1685-1701, 1987. PubMed: 2445894

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rosen H. Role of CR3 in induced myelomonocytic recruitment: insights from in vivo monoclonal antibody studies in the mouse. J. Leukocyte Biol. 48: 465-469, 1990. PubMed: 2230596

Hutchings P, et al. Transfer of diabetes in mice prevented by blockade of adhesion- promoting receptor on macrophages. Nature 348: 639-642, 1990. PubMed: 2250718

Rosen H, Gordon S. Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J. Exp. Med. 166: 1685-1701, 1987. PubMed: 2445894

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.