Dengue virus type 2 (ATCC® VR-1584)

Classification: Flaviviridae, Flavivirus, Dengue virus  /  Product Format: frozen 1 mL per vial

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Classification Flaviviridae, Flavivirus, Dengue virus
Agent Dengue virus type 2
Strain New Guinea C
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Derived by adaptation from in vivo product ATCC® VR-1255™ to LLC-MK2 Derivative cells (ATCC® CCL-7.1™). ATCC® VR-1255™ and ATCC® VR-1584™ have not been compared for sequence or infectivity in in vivo and tissue culture.
Effect on Host CPE, cell aggregation, sloughing, death
Recommended Host LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 6-8 days
Effect on Host CPE, cell aggregation, sloughing, death
Name of Depositor CL Wisseman, University of Maryland School of Medicine; Yale Arbovirus Research Unit; National Institute of Allergy and Infectious Diseases; ATCC®
Source Serum of febrile patient, New Guinea, 1944

Sabin AB. Research on dengue during World War II. Am. J. Trop. Med. Hyg. 1: 30-50, 1952. PubMed: 14903434

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