Trypanosoma cruzi Chagas (ATCC® 50800)

Strain Designations: M/HOM/BR/78/SYLVIO-X10 CL4  /  Depositor: JA Dvorak  /  Biosafety Level: 2

Strain Designations M/HOM/BR/78/SYLVIO-X10 CL4
Application
Vector borne research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Clone 4 derived from strain SYLVIO-X10 (= ATCC® 50823™)
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain no
Comments
Flow cytometric analysis
Medium ATCC® Medium 1029: LIT medium
Growth Conditions
Temperature: 25°C
Culture System: Axenic
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).  The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after thawed.
  9. Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 mL ATCC medium 1029. 
  10. Incubate at 25°C with the cap screwed on tightly.
  11. Maintain as described above. 
Name of Depositor JA Dvorak
Special Collection NCRR Contract
Chain of Custody
ATCC <-- JA Dvorak <-- M.A. Miles <-- F.T. Silveira
References

Dvorak JA, et al. Trypanosoma cruzi: flow cytometric analysis. I. Analysis of total DNA/organism by means of mithramycin-induced fluorescence. J. Protozool. 29: 430-437, 1982. PubMed: 6182288

Miyahira Y, Dvorak JA. Kinetoplastidae display naturally occurring ancillary DNA-containing structures. Mol. Biochem. Parasitol. 65: 339-349, 1994. PubMed: 7969274

Scott DA, et al. In situ compositional analysis of acidocalcisomes in Trypanosoma cruzi. J. Biol. Chem. 272: 28020-28029, 1997. PubMed: 9346954

Postan M, et al. Studies of Trypanosoma cruzi clones in inbred mice. III. Histopathological and electrocardiographical responses to chronic infection. Am. J. Trop. Med. Hyg. 37: 541-549, 1987. PubMed: 3318521

Nozaki T, Dvorak JA. Trypanosoma cruzi: flow cytometric analysis of developmental stage differences in DNA. J. Protozool. 38: 234-243, 1991. PubMed: 1880761

Postan M, et al. Studies of Trypanosoma cruzi clones in inbred mice. I. A comparison of the course of infection of C3H/HEN- mice with two clones isolated from a common source. Am. J. Trop. Med. Hyg. 32: 497-506, 1983. PubMed: 6407346