Plasmodium falciparum Welch (ATCC® 30932)

Strain Designations: FCR-3/FMG [ FCR-3/Gambia)]  /  Depositor: W Trager  /  Biosafety Level: 2

Strain Designations FCR-3/FMG [ FCR-3/Gambia)]
Emerging infectious disease research
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Human clinical specimen, Fajara Gambia, 1976
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain no
Medium ATCC® Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37°C
Culture System: In vitro culture in human erythrocytes
Cryopreservation Reagents
Glycerolyte medium**
Glycerol: 57.00 g
NaC3H5O3 (Sodium lactate): 1.60 g
KCl: 30.00 mg
NaH2PO4: 1.38 g
Glass distilled water: 100.00 mL
Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave and store at 4°C.

Harvesting and Preservation
Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.
  1. Centrifuge ring-stage culture for 5 mins at 1000 x g in 50 mL centrifuge tube.
  2. Aspirate supernatant using sterile Pasteur pipet.
  3. Resuspend pellet gently in remaining supernatant.
  4. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  5. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.).
  6. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor W Trager
Special Collection ATCC
Year of Origin 1976

Isolated from an infected human blood sample collected by M. Friedman, Medical Research Council Labs Clinic, Fajara, Gambia, 1976.

Jensen JB, et al. Clinical drug-resistant falciparum malaria acquired from cultured parasites. Am. J. Trop. Med. Hyg. 30: 523-525, 1981. PubMed: 7020443

Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27: 743-746, 1978. PubMed: 356635

Jensen JB, Trager W. Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J. Parasitol. 63: 883-886, 1977. PubMed: 335035