Canine coronavirus (ATCC® VR-809)

Classification: Nidovirales, Coronaviridae, Coronavirus, Group 1  /  Product Format: frozen

Classification Nidovirales, Coronaviridae, Coronavirus, Group 1
Agent Canine coronavirus
Strain 1-71
Common Name Canine coronavirus
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments
Antigenically related to transmissible gastroenteritis virus of swine
This preparation has tested positive for Mycoplasma contamination
Effect on Host
CPE, cell rounding and monolayer degeneration
Recommended Host
A-72 cells (ATCC® CRL-1542™)
Growth Conditions

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% -90% confluent (not 100% confluent).

Incubation: 1 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended.

Effect on Host
CPE, cell rounding and monolayer degeneration
Name of Depositor LN Binn
Special Collection ATCC
Source Feces from dog with diarrhea
References

Binn LN, et al. Recovery and characterization of a coronavirus from military dogs with diarrhea. Proc. 78th Ann. Meeting U.S. Anim. Health Assoc. 78: 359-366, 1974. PubMed: 4377955