Bovine adenovirus 3 (ATCC® VR-639)

Classification: Adenoviridae, Mastadenovirus, Bovine adenovirus B  /  Product Format: frozen

Classification Adenoviridae, Mastadenovirus, Bovine adenovirus B
Deposited As Bovine adenovirus type 3
Agent Bovine adenovirus 3
Strain WBR-1
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Conjunctiva from a healthy cow
Product Format frozen
Storage Conditions  -70°C or colder
Comments
70-80 nm in diameter. Characteristic adenovirus structure. Shares common group CF antigen with human adenoviruses. Fairly stable at -70C. No cross-reaction with bovine adenovirus types 1 and 2. Antigenicity good. Acid stable.
70-80 nm in diameter. Characteristic adenovirus structure. Shares common group CF antigen with human adenoviruses. Fairly stable at -70C. No cross-reaction with bovine adenovirus types 1 and 2. Antigenicity good. Acid stable.
Effect on Host
Yes, in vitro effects: Characteristic adenovirus CPE and Type B intranuclear inclusion bodies
 CPE, characteristic adenovirus CPE and Type B intranuclear inclusion bodies
Recommended Host
primary bovine embryonic kidney cells

Production Host:  BT (ATCC® CRL-1390)

primary bovine embryonic kidney cells in tissue culture; BT cells (ATCC CRL-1390)
Growth Conditions
Duration: 3-4 days at 37C, 5% CO2
Effect on Host
Yes, in vitro effects: Characteristic adenovirus CPE and Type B intranuclear inclusion bodies
 CPE, characteristic adenovirus CPE and Type B intranuclear inclusion bodies
Name of Depositor SB Mohanty
Source
Conjunctiva from a healthy cow
References

Darbyshire JH, et al. A new adenovirus serotype of bovine origin. J. Comp. Pathol. 75: 327-330, 1965. PubMed: 4285369