Bovine adenovirus 3 (ATCC® VR-639)

Classification: Adenoviridae, Mastadenovirus, Bovine adenovirus B  /  Product Format: frozen

Permits and Restrictions

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Classification Adenoviridae, Mastadenovirus, Bovine adenovirus B
Deposited As Bovine adenovirus type 3
Agent Bovine adenovirus 3
Strain WBR-1
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Conjunctiva from a healthy cow
Product Format frozen
Storage Conditions  -70°C or colder
Comments
70-80 nm in diameter. Characteristic adenovirus structure. Shares common group CF antigen with human adenoviruses. Fairly stable at -70C. No cross-reaction with bovine adenovirus types 1 and 2. Antigenicity good. Acid stable.
70-80 nm in diameter. Characteristic adenovirus structure. Shares common group CF antigen with human adenoviruses. Fairly stable at -70C. No cross-reaction with bovine adenovirus types 1 and 2. Antigenicity good. Acid stable.
Effect on Host
Yes, in vitro effects: Characteristic adenovirus CPE and Type B intranuclear inclusion bodies
 CPE, characteristic adenovirus CPE and Type B intranuclear inclusion bodies
Recommended Host
primary bovine embryonic kidney cells

Production Host:  BT (ATCC® CRL-1390)

primary bovine embryonic kidney cells in tissue culture; BT cells (ATCC CRL-1390)
Growth Conditions
Duration: 3-4 days at 37C, 5% CO2
Effect on Host
Yes, in vitro effects: Characteristic adenovirus CPE and Type B intranuclear inclusion bodies
 CPE, characteristic adenovirus CPE and Type B intranuclear inclusion bodies
Name of Depositor SB Mohanty
Source
Conjunctiva from a healthy cow
References

Darbyshire JH, et al. A new adenovirus serotype of bovine origin. J. Comp. Pathol. 75: 327-330, 1965. PubMed: 4285369

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation