Bovine adenovirus 1 (ATCC® VR-313)

Classification: Adenoviridae, Mastadenovirus, Bovine mastadenovirus A  /  Product Format: frozen 1.0 mL

Classification Adenoviridae, Mastadenovirus, Bovine mastadenovirus A
Deposited As Bovine adenovirus type 1
Agent Bovine adenovirus 1
Strain 10
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Feces of normal calf
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-313 images
Comments REFERENCE STRAIN: Pereira HG et al. A short description of the Adenovirus group. Virology 20: 613-620, 1963.

Agglutinates rat RBC. CPE may be minimal, frequently demonstrating less than 25% involvement of cell sheet, even with prolonged incubation.  AAV reported to be present.
Effect on Host
CPE, cell rounding with vacuolization, clumping, and sloughing
Recommended Host
MDBK (NBL-1) cells (ATCC® CCL-22™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding with vacuolization, clumping, and sloughing
Name of Depositor M Klein
Source Feces of normal calf
References

Klein M, et al. Isolation from cattle of a virus related to human adenovirus. Proc. Soc. Exp. Biol. Med. 102: 1-4, 1959. PubMed: 14409689

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828