Canine parainfluenza virus (ATCC® VR-1573)

Classification: Paramyxoviridae, Paramyxovirinae, Unclassified  /  Product Format: frozen

Classification Paramyxoviridae, Paramyxovirinae, Unclassified
Agent Canine parainfluenza virus
Strain 78-238
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
This an encephalitogenic strain of Canine parainfluenza virus.
Effect on Host
Yes, in vitro effects: cytopathic effect, syncytial sloughing
CPE, syncytial sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:┬á ATCC® CCL-81™ (Vero)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 36.0°C
Duration: 3-4 days; For best results cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vitro effects: cytopathic effect, syncytial sloughing
CPE, syncytial sloughing
Name of Depositor ATCC, JF Evermann
Special Collection ATCC
Source
derived from existing strain
References

Evermann JF, et al. Isolation of a paramyxovirus from the cerebrospinal fluid of a dog with posterior paresis. J. Am. Vet. Med. Assoc. 177: 1132-1134, 1980. PubMed: 6254938

Evermann JF, et al. Properties of an encephalitogenic canine parainfluenza virus. Arch. Virol. 68: 165-172, 1981. PubMed: 6168243

or colder

ATCC VR-963 was filtered to remove bacterial and fungal contaminants and reaccessioned as ATCC VR-1573. ATCC VR-963 was isolated from cerebrospinal fluid from dog with posterior paresis.

or colder