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Species
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Hepatitis delta virus, Hepatitis delta virus
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Applications
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Blood borne research Sexually Transmitted Disease Research
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Vector
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Construct size (kb): 9.800000190734863
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Insert
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DNA: cDNA Insert lengths(kb): 5.099999904632568 Tissue: DNA from liver of an infected woodchuck Gene product: delta antigen Target Gene: delta antigen
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Shipping Information
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Shipped: Freeze dried Escherichia coli containing the plasmid.
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.7, 2.1, 1.65 (triplet); EcoRI--2.7, 2.1, 1.65 (triplet); PstI--4.0, 2.4, 1.3 (doublet), 0.5; BglI--2.5, 1.65 (doublet), 1.5, 1.4, 1.1; BglII--6.5, 1.65 (doublet). This is plasmid in E.coli that can be used to transfect COS-7 cells. The unit genome contains the following restriction sites (bp from the 5' end): BglI--716; BglII--224; EcoRI--1427; HincII--962, 1200; NcoI--576; PstI--651, 1083; SalI--962; SmaI--479, 1107; XbaI--781; XhoI--1272, 1506, 1545. Virus DNA cloned after passage in liver of woodchuck The insert consists of a head to tail trimer of the full length genome (1.679 kb) beginning at the unique EcoRI site and oriented so that transcription from the SV40 late promoter gives genomic RNA. Excising with BamHI+EcoRI yields 3 insert fragments of 1.7 kb and vector fragments of 1.8 kb and 3.0 kb. The construct has a unique BamHI site. Transfected COS-7 cells replicate the HDV RNA genome, and produce the 195 aa small form of the delta antigen, encoded from nt 1598 to 1014.
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References
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Kuo MY, et al. Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen. J. Virol. 63: 1945-1950, 1989. PubMed: 2649689
Kuo MY, et al. Molecular cloning of hepatitis delta virus RNA from an infected woodchuck liver: sequence, structure, and applications. J. Virol. 62: 1855-1861, 1988. PubMed: 3367426
John M Taylor, personal communication
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