Detroit 532 (ATCC® CCL-54)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number 47; trisomic group 21; Down Syndrome
Clinical Data
male
Caucasian
Comments
Cells senesce after approximately 30 serial subcultivations counted from the tissue of origin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium:
  • lactalbumin hydrosolate 0.1% (final conc.)
  • fetal bovine serum 10% (final conc.)
  • Subculturing
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37.0°C
    STR Profile
    Amelogenin: X,Y
    CSF1PO: 12
    D13S317: 11,13
    D16S539: 10,13
    D5S818: 9,12
    D7S820: 8
    THO1: 7,9
    TPOX: 8
    vWA: 16,18
    Isoenzymes
    G6PD, B
    Name of Depositor CS Stulberg
    Year of Origin 1964
    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation