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DMS 53 (ATCC® CRL-2062™)

Organism: Homo sapiens, human  /  Tissue: lung  /  Disease: carcinoma; small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease carcinoma; small cell lung cancer
Age 54 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung.
The patient had not received prior therapy.
Clinical Data
54 years
Caucasian
male
Antigen Expression
Leu 7; My23
Receptor Expression
bombesin, expressed
epidermal growth factor (EGF), expressed
transforming growth factor beta (TGF beta), expressed
acetylcholine, expressed
Genes Expressed
adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; human chorionic gonadotropin (hCG); glucagon; growth hormone; 17 beta estradiol; thyroid releasing hormone; oxytocin - neurophysin (OT-NP); parathormone;,somatostatin-like immunoreactivity (SRIF)
Cellular Products
adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; human chorionic gonadotropin (hCG); glucagon; growth hormone; 17 beta estradiol; thyroid releasing hormone; oxytocin - neurophysin (OT-NP); parathormone;
somatostatin-like immunoreactivity (SRIF)
Tumorigenic Yes
Effects
Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments
The patient had not received prior therapy.

The cells express HLA class I and class II antigens.

Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin derived products.

 
Complete Growth Medium Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Interval: Keep cells heavy and subculture often
Subcultivation Ratio: 1:2 to 1:3
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Growth Conditions: Keep cells heavy and subculture often.
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 12,13
D5S818: 10,11
D7S820: 8,11
THO1: 8,9.3
TPOX: 12
vWA: 15,17
Name of Depositor OS Pettengill, G Sorenson
Passage History
Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin derived products.
Year of Origin 1974
References

Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
Restrictions

Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
References

Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760