MSTO-211H (ATCC® CRL-2081)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: pleural effusion  /  Disease: biphasic mesothelioma

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: pleural effusion
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease biphasic mesothelioma
Age 62 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 72; range = 70 to 78
Derivation
The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung.
Derived from metastatic site, lung
Clinical Data
The patient had not received prior radiation or chemotherapy.
62 years
Caucasian
male
The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung.
Oncogene c-myc +; v-src +; v-abl +; v-erb B +; c-raf 1 +; H-ras +; K-ras +; N-ras +; N-myc -; L-myc -; c-myb -; c-fos -; v-fes -; v-fms -; v-sis -
Tumorigenic Yes
Effects
Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells
Comments

The patient had not received prior radiation or chemotherapy.

MSTO-211H cells have high affinity binding sites for EGF, and express Neuron specific enolase (NSE) and both the alpha and beta subunits of human chorionic gonadotropin (HCG).

L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.

The cells overexpress the c-myc protooncogene, and no gene rearrangement or amplification was observed.

They are positive for the expression of v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras.
Expression of N-myc, L-myc, c-myb, c-fos, v-fes, v-fms and v-sis oncogene expression was not detected.

The cells can reach a saturation density of 400000 cells per sq cm, but will slough off of the surface as they attain this density.




Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 13
D5S818: 12
D7S820: 8,12
THO1: 8,9.3
TPOX: 11
vWA: 16,18
Population Doubling Time 20 hrs
Name of Depositor G Bepler
Year of Origin 1985
References

Bepler G, Koehler A. Multiple chromosomal aberrations and 11p allelotyping in lung cancer cell lines. Cancer Genet. Cytogenet. 84: 39-45, 1995. PubMed: 7497441

Bepler G, et al. Characterization of the state of differentiation of six newly established human non-small-cell lung cancer cell lines. Differentiation 37: 158-171, 1988. PubMed: 2840315

Haeder M, et al. Epidermal growth factor receptor expression in human lung cancer cell lines. Cancer Res. 48: 1132-1136, 1988. PubMed: 2830015

Lung Cancer 4: 155-161, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Bepler G, Koehler A. Multiple chromosomal aberrations and 11p allelotyping in lung cancer cell lines. Cancer Genet. Cytogenet. 84: 39-45, 1995. PubMed: 7497441

Bepler G, et al. Characterization of the state of differentiation of six newly established human non-small-cell lung cancer cell lines. Differentiation 37: 158-171, 1988. PubMed: 2840315

Haeder M, et al. Epidermal growth factor receptor expression in human lung cancer cell lines. Cancer Res. 48: 1132-1136, 1988. PubMed: 2830015

Lung Cancer 4: 155-161, 1988.