Chlamydia trachomatis (ATCC® VR-879™) Classification: Chlamydiaceae, Chlamydia / Product Format: frozen 1 mL per vial General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Classification Chlamydiaceae, Chlamydia Deposited As Trachoma serotype h Agent Chlamydia trachomatis Strain Trachoma type H strain UW-43/Cx Biosafety Level 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Product Format frozen 1 mL per vial Storage Conditions -70°C or colder Comments Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 rpm (750 x g) for one hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg /mL cycloheximide. Incubate at 36°C for 3 days. Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa Recommended Host McCoy cells (ATCC® CRL-1696™) Growth Conditions Temperature: 36°C Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). Incubation: 3 days, a 5% CO2 in air atmosphere is recommended Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa Name of Depositor SP Wang Source Human cervix (epithelial tissue), cervicitis, Seattle, WA, 1971 References Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS