Comments
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Centrifugation (2000 X g for 1 hr.) and addition of cycloheximide recommended for passage in cell culture. *Requests for this agent must carry signed statement assuming all risks and responsibility for lab handling. Triturate fluid several times to disperse chlamydia. For cell culture, centrifuge inoculumn, adsorb for 1 hour and feed with HEPES buffered culture medium.
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Effect on Host
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Cytopathic effects (inclusions) in tissue culture Death of embryo
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