Chlamydia trachomatis Serovar F (ATCC® VR-346)

Classification: Chlamydiaceae  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae
Agent Chlamydia trachomatis Serovar F
Strain IC-Cal-3
Applications
Sexually Transmitted Disease Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

Rapid loss in titer when stored above -70°C. Activities with high potential for aerosol production require BSL 3 facilities and practices.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies
Recommended Host
McCoy cells (ATCC CRL-1696)
Growth Conditions Temperature: 35.5°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 95-100% confluent. Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). Adsorb by centrifugation at 2100 x g for 1 hour at 20°C. End adsorption by adding growth medium.
Incubation: 3 days
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies
Name of Depositor L Hanna, E Jawetz, Centers for Disease Control and Prevention
Source Eye of nine-day-old baby, California, 1960
References

Hanna L, et al. Isolation of viruses from inclusion conjunctivitis in the newborn. Am. J. Ophthamol. 53: 774-780, 1962. PubMed: 13904377