Simkania negevensis Everett et al. (ATCC® VR-1471)

Classification: Simkaniaceae  /  Product Format: frozen

Classification Simkaniaceae
Agent Simkania negevensis Everett et al.
Strain Z
Applications
Host of choice: Vero cells (ATCC CCL-81)
Biosafety Level 2
Isolation
contaminated Vero cells, passaged in Israel
Product Format frozen
Storage Conditions -70°C or colder
Comments
Antigenic cross-reactivity by immunoblot with chlamydial heatshock proteins
Effect on Host
Cytopathic effects (black granulated cytoplasm, "tar spots", developing to numerous vacuoles, with development of "dancing" particles)
Black granulated cytoplasm ("tar spots"), developing to numerous vacuoles, with development of "dancing" particles.
Recommended Host
cells of human and primate origin
Vero (ATCC CCL-81)
Growth Conditions
Incubation: 5 days at 35°C to 37°C in Vero cells (ATCC CCL-81)
Effect on Host
Cytopathic effects (black granulated cytoplasm, "tar spots", developing to numerous vacuoles, with development of "dancing" particles)
Black granulated cytoplasm ("tar spots"), developing to numerous vacuoles, with development of "dancing" particles.
Name of Depositor M Friedman
Special Collection NCRR Contract
Source
contaminated Vero cells, passaged in Israel
References

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

Kahane S, et al. Description and partial characterization of a new Chlamydia-like microorganism. FEMS Microbiol. Lett. 109: 329-333, 1993. PubMed: 8339923

type strain

Cross References

Nucleotide (GenBank) : AJ012178 Simkania negevensis partial rnpB gene for RNase P RNA subunit.