Waddlia chondrophila Everett et al. (ATCC® VR-1470)

Classification: Waddliaceae  /  Product Format: frozen

Classification Waddliaceae
Agent Waddlia chondrophila Everett et al.
Strain WSU 86-1044
Applications
Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate).
Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp.
Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496.
Biosafety Level 2
Isolation
pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation
Product Format frozen
Storage Conditions -70°C or colder
Genome Sequenced Strain

Yes

Comments
Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp. and 14 members of the genus Rickettsia all failed to react significantly. Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate).
Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496.
MEM with 10% serum
Effect on Host
Dense forms enter cytoplasm and reticulated forms develop, then change to dense forms and are released from the cell
Inclusions visualized by light microscopy as dense forms.  Some cell death due to lysis.  Cells become rounded and dense.
Recommended Host
bovine turbinate (BT) cells (ATCC CRL-1390)
BT cells (ATCC CRL-1390); P388D1 mouse macrophage cells (ATCC CCL-46)
Growth Conditions
Incubation: 3 days at 35°C in BT cells (ATCC CRL-1390), 5% CO2
Effect on Host
Dense forms enter cytoplasm and reticulated forms develop, then change to dense forms and are released from the cell
Inclusions visualized by light microscopy as dense forms.  Some cell death due to lysis.  Cells become rounded and dense.
Name of Depositor TF McElwain
Special Collection NCRR Contract
Source
pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation
References

Dilbeck PM, et al. Isolation of a previously undescribed rickettsia from an aborted bovine fetus. J. Clin. Microbiol. 28: 814-816, 1990. PubMed: 2185269

Kocan KM, et al. Development of a rickettsia isolated from an aborted bovine fetus. J. Bacteriol. 172: 5949-5955, 1990. PubMed: 2211519

Rurangirwa FR, et al. Analysis of the 16S rRNA gene of micro-organism WSU 86-1044 from an aborted bovine foetus reveals that it is a member of the order Chlamydiales: proposal of Waddliaceae fam. nov., Waddlia chondrophila gen. nov., sp. nov.. Int. J. Syst. Evol. Microbiol. 49: 577-581, 1999. PubMed: 10319478

type strain

Terry F McElwain, personal communication

Cross References

Nucleotide (GenBank) : AF042496 16S ribosomal RNA and 23S ribosomal RNA, complete sequence