Rickettsia japonica Uchida et al. (ATCC® VR-1363)

Classification: Rickettsiae  /  Product Format: frozen

Classification Rickettsiae
Agent Rickettsia japonica Uchida et al.
Strain YH
Applications
Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae.
Biosafety Level 3
Isolation
blood of patient with oriental spotted fever, Japan
Product Format frozen
Storage Conditions Liquid nitrogen freezer
Comments
Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae. Vero cells demonstrated to be mycoplasma-free before infection.
Infected Vero cells grow like uninfected cells in MEM with 10% FBS devoid of antibiotics. Subculture by dispersion with 0.05% trypsin and 0.02% EDTA is possible for many passages.
Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit.
Effect on Host
Cytopathic effects in BHK and L929
No observable CPE in Vero
Death of CE
Fever and scrotal swelling of guinea pig
Recommended Host
Vero cells (ATCC CCL-81)
BHK-21(C-13) cells (ATCC CCL-10); L-929 cells (ATCC CCL-1); chicken embryo; guinea pig
Growth Conditions
Temperature: 34°C
Incubation: 3-6 days
Effect on Host
Cytopathic effects in BHK and L929
No observable CPE in Vero
Death of CE
Fever and scrotal swelling of guinea pig
Name of Depositor T Uchida
Source
blood of patient with oriental spotted fever, Japan
References

Uchida T, et al. Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int. J. Syst. Bacteriol. 42: 303-305, 1992. PubMed: 1581190

Uchida T, et al. Isolation of a spotted fever group Rickettsia from a patient with febrile exanthematous illness in Shikoku, Japan. Microbiol. Immunol. 30: 1323-1326, 1986. PubMed: 3553872

type strain