Dehalococcoides sp. (ATCC® BAA-2098)

Strain Designations: FL2  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations FL2
Isolation
River sediment, Red Cedar River; Okemos, Michigan, USA
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Preceptrol® no
Type Strain no
Comments
Growth can best be monitored using real time PCR; see Protocol for quantifying in Related Links.
Medium ATCC® Medium 2760: MOPS Buffered Medium for Dehalococcoides sp.
Growth Conditions
Temperature: 24-30°C
Atmosphere: Anaerobic; N2-H2 (2-20% H2 in the headspace)
Name of Depositor FE Löffler
Isolation
River sediment, Red Cedar River; Okemos, Michigan, USA
References

Moench TT, Zeikus JG. An improved preparation method for a titanium (III) media reductant. J. Microbiol. Methods. 1: 199-202, 1983.

He J, et al. Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe. Environ. Microbiol. 9: 1442-1450, 2005. PubMed: 16104866

Ritalahti KM, et al. Quantitative PCR targeting 16S rRNA and reductive dehalogenase genes simultaneously monitors multiple Dehalococcoides strains. Appl. Environ. Microbiol. 72(4): 2765-2774, 2006. PubMed: 16597981

Cross References

Nucleotide (GenBank) : AF357918 Dehalococcoides sp. FL2 16S ribosomal RNA gene, partial sequence