Rhizobium leguminosarum Jordan (ATCC® 53912)

Strain Designations: pTFX1::10-15 [10-15 DH5alpha, TA1::10-15, TA1::TFX:Tn5, TFX:Tn5; 10-15, pTFX1::Tn5]  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations pTFX1::10-15 [10-15 DH5alpha, TA1::10-15, TA1::TFX:Tn5, TFX:Tn5; 10-15, pTFX1::Tn5]
Application
Produces protein trifolitoxin, synthesis and resistance genes
Isolation
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol® no
Type Strain no
Comments
Recombinant strain expressing trifolitoxin that is capable of promoting the formation of nitrogen fixation nodules.
Production of, and resistance to, trifolitoxin
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Medium ATCC® Medium 111: Rhizobium X medium
Growth Conditions
Temperature: 26.0°C
Name of Depositor Wisconsin Alumni Res. Fndn., DJ Houser, Wisconsin Alumni Res. Fndn.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Isolation
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
References

Triplett EW. Recombinant Rhizobium bacteria inoculants. US Patent 5,183,759 dated Feb 2 1993

Triplett EW. Isolation of genes involved in nodulation competitiveness from Rhizobium leguminosarum bv. trifolii T24. Proc. Natl. Acad. Sci. USA 85: 3810-3814, 1988.