Gallid herpesvirus 2 (ATCC® VR-624)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Herpesviridae, Mardivirus
Deposited As Marek's disease virus
Agent Gallid herpesvirus 2
Strain GA-5
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

chicken tissue with Marek's disease
Product Format frozen 1 mL per vial
Storage Conditions Vapor phase of liquid nitrogen
Infected CK cell suspension with 7% DMSO + Penicillin/Streptomycin + Fungizone®. Similar to JM strain. Dilution of infected chicken kidney cell suspension with medium 2-3 times the volume after thawing will enhance virus recovery.
Effect on Host
CPE, focal lesions
Recommended Host
chicken kidney cells
Growth Conditions
Temperature: 38.5°C
Incubation: 5-7 days
Effect on Host
CPE, focal lesions
Name of Depositor BW Calnek, KA Schat, Cornell University New York State College of Veterinary Medicine
U.S. Patent
U.S. Patent Number
Chicken tissue with Marek?s disease, Georgia, 1964

Calnek BW, Hitchner SB. Highly potent, viable and stable cell-free virus preparations from cells infected with cell-associated viruses and method for obtaining the same. US Patent 3,783,098 dated Jan 1 1974

Eidson CS, Schmittle SC. Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens. Avian Dis. 12: 467-476, 1968. PubMed: 4301071

Reddy SM, et al. Development of a quantitative-competitive polymerase chain reaction assay for serotype 1 Marek's disease virus. Avian Dis. 44: 770-775, 2000. PubMed: 11195630.

Silva RF. Differentiation of pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDVs) by the polymerase chain reaction amplification of the tandem direct repeats within the MDV genome. Avian Dis. 36: 521-528, 1992. PubMed: 1329706

Cross References

Nucleotide (GenBank) : U63464 Gallid herpesvirus 2 DNA polymerase gene, partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation