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Zika virus (ATCC® VR-1859)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen 1.0 mL per vial

Permits and Restrictions

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Classification Flaviviridae, Flavivirus
Agent Zika virus
Strain H/PAN/2015/CDC-259359
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1859 images
Effect on Host
CPE, rounding and detachment
Recommended Host
VERO C1008 [Vero 76, clone E6, Vero E6] cells (ATCC® CRL-1586™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 5-8 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed throughout monolayer.

Effect on Host
CPE, rounding and detachment
Name of Depositor B Russell, Centers for Disease Control and Prevention
Source Serum specimen collected from a human, Panama, December 18, 2015
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation