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Zika virus (ATCC® VR-1844)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen 1.0 mL per vial

Permits and Restrictions

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Classification Flaviviridae, Flavivirus
Agent Zika virus
Strain FLR
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Comments CPE clearer to observe in VERO C1008 [Vero 76, clone E6, Vero E6] cells (ATCC® CRL-1586™).
Effect on Host
CPE, sloughing
Recommended Host
Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™)
Growth Conditions

Temperature: 28°C

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb for 1 hour at 28°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 4-6 days at 28°C in a humidified 5% CO2 atmosphere, until CPE is progressed throughout monolayer.

Effect on Host
CPE, sloughing
Name of Depositor R Rico-Hesse, Baylor College of Medicine
Source Human serum, Colombia, December 2015
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • For-profit customers must obtain a research use license agreement from the Contributor prior to shipment.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

This material’s use is governed by the MTA and the following restrictions: (1) Prior to purchase, for-profit entities must obtain a research use license from Baylor College of Medicine and (2) when a Commercial Use is contemplated, for-profit and non-profit entities must obtain a commercial use license from Baylor College of Medicine before this item can be shipped. Contact Baylor Licensing Group, by phone: (713) 798-6821 or email: blg@bcm.tmc.edu.