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Zika virus (ATCC® VR-1839)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen 1.0 mL per vial

Permits and Restrictions

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Classification Flaviviridae, Flavivirus
Agent Zika virus
Strain IB H 30656
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1839 images
Effect on Host
CPE, Refractile cell rounding, sloughing, detachment
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 3-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer.

Effect on Host
CPE, Refractile cell rounding, sloughing, detachment
Name of Depositor R Tesh, The University of Texas Medical Branch, Galveston, Texas
Source Human blood, Ibadan, Nigeria, September 9, 1968
Year of Origin 1968
References

Haddow AD, et al. Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage. PLoS Negl. Trop. Dis. 6: e1477, 2012. PubMed: 22389730

McNamara FN. Zika virus: a report on three cases of human infection during an epidemic of jaundice in Nigeria. Trans. R. Soc. Trop. Med. Hyg. 48: 139-145, 1954. PubMed: 13157159

Cross References

Nucleotide (GenBank) : HQ234500 Zika virus isolate IbH_30656 polyprotein gene, partial cds

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation