P3.6.2.8.1 (ATCC® TIB-8)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: plasmacytoma; myeloma

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Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Strain BALB/c
Applications
This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
Tested and found negative for ectromelia virus (mousepox).
Karyotype near tetraploid
Derivation
This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
Antigen Expression
H-2d; PC.1
Genes Expressed
immunoglobulin; monoclonal antibody,H-2d; PC.1
Comments
This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
The cells bear surface immunoglobulin (sIg+).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor P Knopf, A Destree, R Hyman
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Knopf PM, et al. Antibody-induced changes in expression of an immunoglobulin surface antigen. Eur. J. Immunol. 3: 351-359, 1973. PubMed: 4758896

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kimmel CB. Immunoglobun, protein and nucleic acid synthesis in cultured myeloma cells. Exp. Cell Res. 65: 202-208, 1971. PubMed: 4101162

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Knopf PM, et al. Antibody-induced changes in expression of an immunoglobulin surface antigen. Eur. J. Immunol. 3: 351-359, 1973. PubMed: 4758896

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kimmel CB. Immunoglobun, protein and nucleic acid synthesis in cultured myeloma cells. Exp. Cell Res. 65: 202-208, 1971. PubMed: 4101162

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914