P3.6.2.8.1 (ATCC® TIB-8)

Organism: Mus musculus, mouse  /  Cell Type:: B lymphocyte  /  Disease: plasmacytoma; myeloma

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Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease plasmacytoma; myeloma
Strain BALB/c
Applications
This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
Tested and found negative for ectromelia virus (mousepox).
Karyotype near tetraploid
Derivation
This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
Antigen Expression
H-2d; PC.1
Genes Expressed
immunoglobulin; monoclonal antibody,H-2d; PC.1
Cellular Products
immunoglobulin; monoclonal antibody
Comments
This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
The cells bear surface immunoglobulin (sIg+).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor P Knopf, A Destree, R Hyman
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Knopf PM, et al. Antibody-induced changes in expression of an immunoglobulin surface antigen. Eur. J. Immunol. 3: 351-359, 1973. PubMed: 4758896

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kimmel CB. Immunoglobun, protein and nucleic acid synthesis in cultured myeloma cells. Exp. Cell Res. 65: 202-208, 1971. PubMed: 4101162

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Knopf PM, et al. Antibody-induced changes in expression of an immunoglobulin surface antigen. Eur. J. Immunol. 3: 351-359, 1973. PubMed: 4758896

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kimmel CB. Immunoglobun, protein and nucleic acid synthesis in cultured myeloma cells. Exp. Cell Res. 65: 202-208, 1971. PubMed: 4101162

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914