R1.G1 (ATCC® TIB-44)

Organism: Mus musculus, mouse  /  Cell Type:: T lymphocyte  /  Tissue: thymus  /  Disease: lymphoma

Organism Mus musculus, mouse
Tissue thymus
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain C58/J
Applications
This line was derived from R1.1 (ATCC TIB-42).
The cells are resistant to 0.1 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from R1.1 (ATCC TIB-42).
Antigen Expression
H-2k; Thy-1.2; TL
Genes Expressed
H-2k; Thy-1.2; TL
Comments
This line was derived from R1.1 (ATCC TIB-42).
The cells are resistant to 0.1 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor R Hyman
References

Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

Basic Documentation
References

Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.