R1E/TL8x.1 (ATCC® TIB-43™) Organism: Mus musculus, mouse / Cell Type:: T lymphocyte / Tissue: thymus / Disease: lymphoma General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Mus musculus, mouse Tissue thymus Cell Type T lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease lymphoma Strain C58/J Applications Tested and found negative for ectromelia virus (mousepox). Storage Conditions liquid nitrogen vapor phase Antigen Expression H-2k; Thy-1.2 Genes Expressed H-2k; Thy-1.2 Comments Unlike the parental line R1.1, these cells do not express TL antigen.Tested and found negative for ectromelia virus (mousepox). Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Subculturing Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.Medium Renewal: Every 2 to 3 days Cryopreservation Freeze medium: culture medium, 92.5%; DMSO, 7.5%Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37.0°C Name of Depositor R Hyman References Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976. derived from R1.1 (ATCC TIB-42). Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS References Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976. derived from R1.1 (ATCC TIB-42).