EL4.BU.1.OUAr.1.1 (ATCC® TIB-41™) Organism: Mus musculus, mouse / Cell Type:: T lymphocyte / Disease: lymphoma General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Mus musculus, mouse Cell Type T lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease lymphoma Strain C57BL/6N Storage Conditions liquid nitrogen vapor phase Derivation This line was derived from the EL4 cell line (see ATCC TIB-39). Antigen Expression H-2b; Thy-1.2 Comments This line was derived from the EL4 cell line (see ATCC TIB-39).The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain.Tested and found negative for ectromelia virus (mousepox). Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10% Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2-3 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Medium Renewal: Every 2 to 3 days Culture Conditions Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% Name of Depositor R Hyman References Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS References Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.