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B21-2 (ATCC® TIB-229)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody immunoprecipitates polypeptides typical of class II antigens, and competes with alloantisera against Ia-8 (anti I-A b,d) but not Ia-7 (anti I-E d,k) antisera.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against I-A b,d
Cellular Products
immunoglobulin; monoclonal antibody; against I-A b,d
Tumorigenic Yes
Effects
Yes, in irradiated (600R) pristane primed BALB/c mice
Comments
Animals were immunized intravenously with live dendritic cells from BALB/c mice.
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
The antibody immunoprecipitates polypeptides typical of class II antigens, and competes with alloantisera against Ia-8 (anti I-A b,d) but not Ia-7 (anti I-E d,k) antisera.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Name of Depositor RM Steinman
Deposited As rat (B cell); mouse (myeloma)
References

Steinman RM, et al. Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. J. Exp. Med. 152: 1248-1261, 1980. PubMed: 6448907

Nussenzweig MC, et al. Studies of the cell surface of mouse dendritic cells and other leukocytes. J. Exp. Med. 154: 168-187, 1981. PubMed: 7252426

Schuler G, Steinman RM. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J. Exp. Med. 161: 526-546, 1985. PubMed: 3871837

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

Basic Documentation
References

Steinman RM, et al. Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. J. Exp. Med. 152: 1248-1261, 1980. PubMed: 6448907

Nussenzweig MC, et al. Studies of the cell surface of mouse dendritic cells and other leukocytes. J. Exp. Med. 154: 168-187, 1981. PubMed: 7252426

Schuler G, Steinman RM. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J. Exp. Med. 161: 526-546, 1985. PubMed: 3871837

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902