CH1 (ATCC® TIB-221)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: lymphoma

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Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain B10.H-2aH-4bp/Wts(2a4b)
Applications
transfection host
Derivation
This line was established in tissue culture from the CH1 B cell lymphoma.
Antigen Expression
I-Ak; I-Ek
Genes Expressed
immunoglobulin (surface); against E. coli,I-Ak; I-Ek
Cellular Products
immunoglobulin (surface); against E. coli
Comments
This line was established in tissue culture from the CH1 B cell lymphoma.
The cells produce, but do not secrete, immunoglobulin.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml and maintain between 3 x 10 exp5 and 8 x 10 exp5 cells/ml.
Isotype IgM; lambda light chain
Name of Depositor G Haughton
References

Lanier LL, et al. Transplantable B-cell lymphomas in B10. H-2aH-4bp/Wts mice. Immunogenetics 16: 367-371, 1982. PubMed: 6983495

Lanier LL, et al. Novel type of murine B-cell lymphoma. Nature 271: 554-555, 1978. PubMed: 304532

J. Immunol. 121: 2353, 1978.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lanier LL, et al. Transplantable B-cell lymphomas in B10. H-2aH-4bp/Wts mice. Immunogenetics 16: 367-371, 1982. PubMed: 6983495

Lanier LL, et al. Novel type of murine B-cell lymphoma. Nature 271: 554-555, 1978. PubMed: 304532

J. Immunol. 121: 2353, 1978.