P1.17 (ATCC® TIB-10)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Tissue: plasmacytoma; myeloma  /  Disease: plasmacytoma; myeloma

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Organism Mus musculus, mouse
Tissue plasmacytoma; myeloma
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease plasmacytoma; myeloma
Strain BALB/c
Applications
The P1.17 line was established in tissue culture from the ADJPC5 mineral oil induced BALB/c plasmacytoma.
Tested and found negative for ectromelia virus (mousepox).
Karyotype near tetraploid; modal number = 78
Derivation
The P1.17 line was established in tissue culture from the ADJPC5 mineral oil induced BALB/c plasmacytoma.
Antigen Expression
H-2d; PC.1
Genes Expressed
immunoglobulin; monoclonal antibody,H-2d; PC.1
Cellular Products
immunoglobulin; monoclonal antibody
Comments
The P1.17 line was established in tissue culture from the ADJPC5 mineral oil induced BALB/c plasmacytoma.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a; kappa light chain
Name of Depositor K Horibata, AW Harris
References

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914

Schubert D, et al. Immunoglobulin biosynthesis. I. A myeloma variant secreting light chain only. J. Mol. Biol. 38: 253-262, 1968. PubMed: 4180611

Schubert D, Horibata K. Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. J. Mol. Biol. 38: 263-271, 1968. PubMed: 4180612

Schubert D, Cohn M. Immunoglobulin biosynthesis. 3. Blocks in defective synthesis. J. Mol. Biol. 38: 273-288, 1968. PubMed: 4180613

Cotton RG, Milstein C. Letter: Fusion of two immunoglobulin-producing myeloma cells. Nature 244: 42-43, 1973. PubMed: 4582485

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914

Schubert D, et al. Immunoglobulin biosynthesis. I. A myeloma variant secreting light chain only. J. Mol. Biol. 38: 253-262, 1968. PubMed: 4180611

Schubert D, Horibata K. Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. J. Mol. Biol. 38: 263-271, 1968. PubMed: 4180612

Schubert D, Cohn M. Immunoglobulin biosynthesis. 3. Blocks in defective synthesis. J. Mol. Biol. 38: 273-288, 1968. PubMed: 4180613

Cotton RG, Milstein C. Letter: Fusion of two immunoglobulin-producing myeloma cells. Nature 244: 42-43, 1973. PubMed: 4582485