Rosculus sp. (ATCC® PRA-134)

Strain Designations: BSA-02190017  /  Depositor: JC Cole, TA Nerad  /  Biosafety Level: 1

Strain Designations BSA-02190017
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
sediment, saltwater
Virginia, United States
Product Format frozen
Storage Conditions liquid nitrogen vapor phase
Type Strain no
Medium ATCC® Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 20.0°C
Duration: xenic; Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831) and as a food source
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Subcultivation
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Name of Depositor JC Cole, TA Nerad
Special Collection NSF - Protistology
Geographical Isolation Cattleshed Marsh (near Hog Island)
References

sampled from a sparina area; Catleshed Marsh (near Hog Island), eastern shore of Virginia