Capan-2 (ATCC® HTB-80)

Organism: Homo sapiens, human  /  Tissue: pancreas  /  Disease: adenocarcinoma

Permits and Restrictions

View Permits View Restrictions

Organism Homo sapiens, human
Tissue pancreas
Product Format frozen
Morphology polygonal
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma
Age 56 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 69; range = 65 to 74.
This is a pseudotriploid human cell line with the modal chromosome number of 69, occurring in 24% of cells. However, cells having chromosome counts from 65 to 70 also occurred at high frequencies. The rate of cells with higher ploidies was 7%. There were eight marker chromosomes common to most cells. They included paired del(1) (p22), t(8q11q), i(14q), single i(7p) and four others. Two more markers were seen only in some cells. Only one normal X chromosome was found in G-banded preparation. The Y chromosome was not detected in the Q-banded preparation.
Images Cell Micrograph of ATCC HTB-80, Capan-2
Clinical Data
56 years
Antigen Expression
Antigen expression: Blood Type B; Rh+
Genes Expressed

Blood Type B; Rh+, 

The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.

Cellular Products
mucin (apomucin, MUC-1, MUC-2)
Tumorigenic Yes
Yes, in nude mice; forms well differentiated adenocarcinoma consistent with pancreatic carcinoma
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
vWA: 17
AK-1, 1
ES-D, 1
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 2
Name of Depositor J Fogh
Deposited As Homo sapiens
Year of Origin 1975

Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact email:


Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822