SCaBER (ATCC® HTB-3)

Organism: Homo sapiens, human  /  Tissue: urinary bladder  /  Disease: squamous cell carcinoma

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Organism Homo sapiens, human
Tissue
urinary bladder
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease squamous cell carcinoma
Age 58 years
Gender male
Ethnicity Black
Karyotype 2n = 46. The stemline chromosome number is hypotriploid with the 2S component occurring at 9.2%., Eleven markers [t(1;?) del (1)(p13), t(3;?), i(4q), t(8;?), t(9;?), M6, t(1;11), t(21;22), M13, and t(20;?)] and 1 to 3 small chromosome fragments were common to most S metaphases., At least one Y chromosome was always detectable. Number 3 was nullisomic. Neither homogenously staining regions (HSR) nor double minutes (DM) were seen.
Clinical Data
58 years
Black
male
Tumorigenic Yes
Effects
Yes, epidermoid carcinoma forms in nude mice
Comments
Contains the unusual type A isoenzyme of glucose-6-phosphate dehydrogenase.
Complete Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,10
THO1: 7,9
TPOX: 9,11
vWA: 16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, A
GLO-I, 1-2
Me-2, 2
PGM1, 1-2
PGM3, 1-2
Name of Depositor C O'Toole
References

O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

O'Toole C, et al. A cell line (SCABER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707-714, 1976. PubMed: 947851

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

O'Toole C, et al. A cell line (SCABER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707-714, 1976. PubMed: 947851

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047